Bacterial Biofilm Quantification: Using a Crystal Violet Assay to Assess Student-Selected Remedies
Tested Studies in Laboratory Teaching, 2016, Volume 37
Jill E. Callahan & Michael J. Cataldi
Abstract
Biofilms are communities of surface-associated bacteria existing in an extracellular matrix. Within such
protective communities, bacterial species are more resistant to antimicrobial agents than their planktonic
counterparts, often resulting in higher levels of virulence and chronic infection. Staphylococcus aureus is a
hearty biofilm former that has been linked to many infections. In this inquiry-based exercise, students
examine the anti-biofilm properties of natural oil-based soaps by performing a hands-on wet lab adaptation
of the crystal violet quantitative biofilm assay (O'Toole, 1998), using S. aureus. This method is an
important and low-budget tool in assessing natural remedies, a common interest of biology research
students. This is a highly adaptable protocol which can be tailored to a variety of species and test agents.
We use cookies to ensure that we give you the best experience on our website. If you continue to use this site we will assume that you are happy with it.Ok
